Journal: Microbiology Spectrum
Article Title: Anti-bacterial activity of dermcidin in human platelets: suppression of methicillin-resistant Staphylococcus aureus growth
doi: 10.1128/spectrum.03273-24
Figure Lengend Snippet: After platelets co-culture with MRSA, a variety of platelet-derived proteins were significantly changed. The supernatant proteins of platelets co-cultured with or without MRSA for 10 h were collected for quantitative proteomic analysis. P, platelets culture alone; P-M, platelets co-culture with MRSA. ( A ) P-M_vs_P group volcano plots. The fold change and the P -value obtained by t -test were used to draw volcanic plots to show the significant differences between the two groups. Abscissa is the difference multiple (logarithmic transformation with base 2), ordinate is the significance of the difference, P -value (logarithmic transformation with base 10), red dots in the figure are the proteins with significant difference ( P < 0.05), and black dots are the proteins with no difference. ( B ) GO functional enrichment analysis of DEPs in P-M _vs_P group. The abscissa in the graph shows enrichment to GO function classification in biological process (BP); the ordinate (left) represents the number of DEPs under each functional classification, and the ordinate (right) represents the percentage of DEPs under each functional classification in the total number of DEPs. GO, Gene Ontology. ( C ) Enriched KEGG pathways of the DEPs (Top 20) in P-M _vs_P group. The vertical Y-axis represents the pathway. The X-axis represents the number of proteins involved in the corresponding pathway. The digit at the right of each strip represents the richness factor of the corresponding pathway. Color of the bar represents the P value calculated using Fisher’s exact test. KEGG, Kyoto encyclopedia of genes and genomes.
Article Snippet: Finally, the protein samples were sent to Shanghai Zhongke New Life Biotechnology Co. for quantitative proteomic analysis by tandem mass tag (TMT) technology, and the detailed experimental analysis methods are shown in .
Techniques: Co-Culture Assay, Derivative Assay, Cell Culture, Transformation Assay, Functional Assay, Stripping Membranes